Swimming pools of pre-built primers may help generate extensive expression profiles, In particular the place assay sensitivity is critically essential.
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In contrast to oligo(dT) primers, these primers usually are not template particular and they are well suited for DNA synthesis using Klenow fragments with DNA templates or for cDNA synthesis working with reverse transcriptase with mRNA templates.
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Common sequences of Short DNA oligonucleotides are often used as primers for sequencing cloned genes (Sanger sequencing). Choose from our usually utilised sequencing primers to initiate your sequence verification assignments.
In contrast to random primers, oligo (dT) primers usually are not suited to degraded samples but are fantastic for setting up cDNA libraries and advised for use with reverse transcriptases (RTs) as Superscript IV RT.
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Swimming pools of pre-developed primers can help generate comprehensive expression profiles, Particularly where by assay sensitivity is critically critical.
Help Minimal complexity regions are some regions inside a DNA sequence that have biased base compositions like a extend of ACACACACACACACACACA. Inner hybridization read more oligo parameters
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